| Actor/Actress |
Role |
| Steve Carell |
Gru (voice) |
| Kristen Wiig |
Lucy (voice) |
| Benjamin Bratt |
Eduardo/ El Macho (voice) |
| Miranda Cosgrove |
Margo (voice) |
| Dana Gaier |
Edith (voice) |
| Elsie Fisher |
Agnes (voice) |
| Russell Brand |
Dr. Nefario (voice) |
| Ken Jeong |
Floyd (voice) |
| Steve Coogan |
Silas (voice) |
| Moisés Arias |
Antonio (voice) |
| Nasim Pedrad |
Jillian (voice) |
| Kristen Schaal |
Shannon (voice) |
| Pierre Coffin |
Kevin the Minion / Bob the Minion / Stuart the Minion / Additional Minions / Evil Minions (voice) |
| Chris Renaud |
Additional Minions / Evil Minions / Italian Waiter (voice) |
| Vanessa Bayer |
Flight Attendant (voice) |
| Ava Acres |
Additional Voices (voice) |
| Lori Alan |
Additional Voices (voice) |
| Jack Angel |
Additional Voices (voice) |
| Eva Bella |
Additional Voices (voice) |
| John Cygan |
Additional Voices (voice) |
| Debi Derryberry |
Additional Voices (voice) |
| Jess Harnell |
Additional Voices (voice) |
| Danny Mann |
Additional Voices (voice) |
| Mona Marshall |
Additional Voices (voice) |
| Mickie McGowan |
Additional Voices (voice) |
| Alec Medlock |
Additional Voices (voice) |
| Laraine Newman |
Additional Voices (voice) |
| Jan Rabson |
Additional Voices (voice) |
| Katie Silverman |
Additional Voices (voice) |
| Jim Ward |
Additional Voices (voice) (as James Kevin Ward) |
| April Winchell |
Additional Voices (voice) |
| Bailey Gambertoglio |
Little Girl #2 (voice) (uncredited) |
| Sherry Lynn |
Additional Voices (uncredited) |
| Nickolai Stoilov |
Arctic Lab Guards (voice) |
| Georgia Cook |
Additional Voices (voice) |
| Mason McNulty |
Additional Voices (voice) |
| Andre Robinson |
Additional Voices (voice) |
| Casey Simpson |
Additional Voices (voice) |
| Claira Nicole Titman |
Additional Voices (voice) (as Claira Titman) |
